HETEROLOGOUS HYBRIDISATION PROTOCOL

[marcvdc at lmb1.rug.ac.be]

I need to do hybridisations with probes of about 100 bp and 20 bp on 
heterologous target dna's.  The expected HOMOLOGY between the probes and the 
target is about 60%.  I realise that the 20 bp probe probably won't work but 
there has to be a nice PROTOCOL for the 100 bp probe.  I have to do 
this by colony hybridisation,  so I will have a lot DNA on my blots (HYBOND N 
nylon membranes (AMERSHAM)).  As a possitive control I will use the homologous 
target.   Is it possible to discriminate the homologous target and the 
heterologous target just by using two consecutive washing steps?  

DOES ANYONE HAS A NICE HETEROLOGOUS HYBRIDISATION PROTOCOL FOR ME ?

OR IS THERE SOMOWHERE ON INTERNET A LIBRARY WHICH CONTAINS THIS KIND OF 
PROTOCOLS?


THANK YOU IN ADVANCE FOR YOUR REPLY



MARC VAN DE CRAEN 
E-MAIL : MARCVDC at LMB1.RUG.AC.BE