Inosine in PCR primers

Chrystala Constantinidou C.Constantinidou at bham.ac.uk
Tue Dec 20 07:05:53 EST 1994


Hello one and all

Does anyone have experience of using inosine in a PCR primer. I have read
that it is not a good idea to have them too close to the 3' end - has
anyone used inosine and how close is too close. What is it about inosine
that may make it unsuitable to be directly at the 3' end?

Thanks for any help

Wig.



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