Maxipep "problem"

seppen at seppen at
Tue Dec 20 05:31:53 EST 1994

Maxiprep problem?
The last weeks we have observed some weird bands in our maxipreps.
If we apply some of our maxipreps without restiction on a agarose gel a faint 
band of about 1 kb is seen. It is really a band and not a RNA smear. Restriction
of the vector removes the band. The maxipreps were grown fresh from a single
Is it possible that the bacterial host strain is contaminated with something?
Any suggestions are very welcome.
Jurgen Seppen
Academic Medical Center
The Netherlands

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