Maxipep "problem"

seppen at amc.uva.nl seppen at amc.uva.nl
Tue Dec 20 05:31:53 EST 1994


Maxiprep problem?
The last weeks we have observed some weird bands in our maxipreps.
If we apply some of our maxipreps without restiction on a agarose gel a faint 
band of about 1 kb is seen. It is really a band and not a RNA smear. Restriction
of the vector removes the band. The maxipreps were grown fresh from a single
colony.
Is it possible that the bacterial host strain is contaminated with something?
Any suggestions are very welcome.
Thanks, 
Jurgen Seppen
Academic Medical Center
Amsterdam
The Netherlands



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