Sequencing question*********:)

Daniel Michael Lasser dml1 at
Tue Dec 20 17:30:06 EST 1994

Dear sorry gel-runner:

Your woes may be due to bubbles UNDER the gel (not in).
For instance, if you are using the IBI sequencer, you need to blow
out all the bubbles which form between the glass plates under your gel
where the bottom spacer was removed.
We use a curved injection needle (18 gauge) on a syringe and inject 
running buffer forcefully to remove all bubbles.
You will see dramatic drop in current and no more smiley faces!
Alternatives exist: use a blotting paper strip as a spacer, do not
remove during the run.

Dan Lasser, Columbia U.

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