request hi eff transient transfection system
skshaw at fas.harvard.edu
Tue Dec 20 17:32:25 EST 1994
Oops, sorry, I omitted the message.
I'm looking for a high efficiency mammalian transient transfection
system. to express a cell surface heterodimer.
High effiency=high percentage of positive cells (>40%)
High levels of expression
I'm currently using COS-7, AprM8 vector, and DEAE-dextran/DMSO shock.
I've tried to optimize conditions re. confluency of COS cells prior to
tx, amounts of DNA, times of incubation, DMSO shock, harvesting.
Effeciency varies from 15-60% positive, although all the factors
mentioned help reduce the number of poor tx.
Please send your imporvements/alternate systems/references. I will post a
skshaw at husc.harvard.edu
More information about the Methods