2-D gel analysis

Micheal McCann mmccann at sju.edu
Tue Dec 20 14:05:50 EST 1994


I have a couple of questions about analyzing autoradiograms of 35-S labeled proteins seperated by 2-Dimensional PAGE.  What I would like to do is to quantitate the amount of label incoporated in various spots and compare these levels between gels.  My questions are: 1) what is the best (and preferably cheapest) software to do such analysis (I already have a 1200 dpi flat bed scanner), 2) I've been told that I must pre-flash the film to get a linear response.  Is this tru and if so, how do I do it?  Please e-mail responses to me at mmccann at sju.edu.  Thank you for your help in this matter.Mike McCannmmccann at sju.edu



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