cleaning PCR primers

Steven Goldberg goldberg at bms.com
Tue Dec 20 14:37:51 EST 1994


 >Another precipitation procedure that's worked well for us is to resuspend your crude pellet in ca. 100 ul dH20, then add MgCl2 to 10 mM final concentration + 5 vol EtOH.  Centrifuge 5 min in a microfuge
wash with 70% EtOH, dry pellet, and resuspend as desired.
> 




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