precise quantificatin of genomic DNA from different sources

schupp at nauvax.ucc.nau.edu schupp at nauvax.ucc.nau.edu
Tue Dec 20 09:49:24 EST 1994


Brian,
        Small amounts of contaminants that are not reflected in the 260/280
ratio, which only reflects protein contamination, can drastically affect the
260 OD.  If the ethidium staining is uniform and the UV irradiation from your
transilluminator is uniform, definitely trust the agarose gel.  If you have
transilluminator is uniform and, also, you are certain there is no intact
RNA remaining in your sample, definitely trust the agarose gel.  Hoescht dye
staining with fluorometry is a good way to go also.  Hope this helps.

Jim Schupp



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