leach at acs.bu.edu
Tue Dec 20 09:46:30 EST 1994
In article <3d64pq$63i at amcnix.amc.uva.nl>, seppen at amc.uva.nl wrote:
> Maxiprep problem?
> The last weeks we have observed some weird bands in our maxipreps.
> If we apply some of our maxipreps without restiction on a agarose gel a faint
> band of about 1 kb is seen. It is really a band and not a RNA smear. Restriction
> of the vector removes the band. The maxipreps were grown fresh from a single
> Is it possible that the bacterial host strain is contaminated with something?
> Any suggestions are very welcome.
Are you sure it isnt supercoiled plasmid?
..... Martin Leach Email:leach at mbcrr.harvard.edu
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