Synthetic Peptide Antibodies
RNeubig at umich.edu
Wed Dec 21 08:40:51 EST 1994
shark at sam.neosoft.com (John D. Valentich) wrote:
> We have recently started preparing polyclonal antibodies in rabbits against synthetic peptides
> from annexins and phospholipase A2 activating protein (PLAP). Peptides were
> conjugated to the carrier BSA. We are wondering about the problem of
> antibodies generated against BSA reacting with serum albumin or albumin-like
> epitopes in other cellular proteins. Questions:
> 1. Is it likely (or inevitable) that our antisera will contain high titer
> antibodies against BSA? Could they be expected to cross react with other
> non-albumin cellular proteins?
They will almost certainly react with albumin.
> 2. If so, is the best (or only?) solution to this problem affinity purification of annexin or PLAP
> antibodies from the antisera?
Affinity purification should work. You could also adsorb the antisera
with BSA and hope to remove many of the cross-reacting antibodies.
It's probably too late now but you can use keyhole limpet hemocyanin
(KLH) as a carrier.
> 3. What are other possible solutions?
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