site-directed mutagenesis and PCR

Gerri Newfry gnew at orion.it.luc.edu
Wed Dec 21 12:35:02 EST 1994


HI!

I have been working on SDM/PCR for a little while now.  I've finally 
amplified two products that when denatured and mixed with each other will 
hopefully combine.  I need to extend each of the products and then do 
another PCR to amplify the mutant.

I want to know if I can use Taq to extend the products, and then just 
proceed with my PCR.  Can I just set everything up as if I'm doing a PCR??

--
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		gerri...gnew at orion.it.luc.edu				   




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