site-directed mutagenesis and PCR
Gerri Newfry
gnew at orion.it.luc.edu
Wed Dec 21 12:35:02 EST 1994
HI!
I have been working on SDM/PCR for a little while now. I've finally
amplified two products that when denatured and mixed with each other will
hopefully combine. I need to extend each of the products and then do
another PCR to amplify the mutant.
I want to know if I can use Taq to extend the products, and then just
proceed with my PCR. Can I just set everything up as if I'm doing a PCR??
--
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gerri...gnew at orion.it.luc.edu
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