riboprobes with biotin

Morag Lancaster morag at uniwa.uwa.edu.au
Thu Dec 22 13:00:03 EST 1994

Hi There,

has anyone had experience making riboprobes using SP6 or T7, incorporating 
biotinylated UTP?  I have been trying to produce a 0.3kb riboprobe but find 
that I either get very little or no product when observed on a gel.  Using 
normal UTP I observe a lot of product.  Increasing the amount of enzyme helps 
but is still not very efficient.


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