cycle sequencing

Piroska Rakoczy rakoczy at uniwa.uwa.edu.au
Wed Dec 21 22:13:02 EST 1994


Has anyone experienced smeary sequencing pattern? We have used PRISM
Dydeoxy Sequencing kit and AB automatic sequencer in an attempt to
sequence an 800bp long PCR product. All we had was a smear.
Thanks for ant hints.

Piroska Rakoczy



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