puting biotin on oligios

NEAL MUSTO nmusto01 at gumedlib.dml.georgetown.edu
Wed Dec 21 09:40:30 EST 1994


Marc:
US Biochemicals has a system where you can add a biotin to the 5" end of
an oligo (pre-existing).
Basically you use ATP gamma S and T4 kinase to add a sulfur group. This
then is reacted with a biotinylating reagent N- iodoacetyl -N-
Biotinylhexenediamine to get a biotin on the end.

Neal A. Musto
Cell Biology
Georgetown University
nmusto at gumedlib.dml.georgetown.edu



More information about the Methods mailing list