Mouse liver DNA?
Lee H. Remington
lremington at popmail.ucsd.edu
Wed Dec 21 07:00:46 EST 1994
I am trying to make genomic DNA from mouse liver. Unfortunately, after
prot K digestion of homogenized tissue and phenol/Chloroform
extraction/ethanol precipitation, I am getting a flocculent white
precipitate coming out with the DNA. I am not seeing the nice stringy
genomic DNA in my precipitation that I expect. I have tried multiple
phenol/chloroform extractions and precipitations but this white junk won't
disappear. Any idea what this precipitate is? lipoprotein? what can be
done to get rid of it? Maybe there is a better part of the mouse to make
DNA from? I need lots for multiple southerns (15 µg/ southern).
Any input would be greatly appreciated!
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