Pcr fragments labelling

Shiao Y. Wang sywang at whale.st.usm.edu
Fri Dec 23 12:04:30 EST 1994


thierry berthe (tberthe at gandalf.crihan.fr) wrote:
: What is the best method to label small Pcr fragments (random priming???). I 
: would like to use them for a northern blot.

Incorporate radiolabeled nucleotide during PCR. Leave out the cold nucleotide.
A paper on this was published in Anal. Biochem. in 1994 I think. Anyone
have the reference?

Shiao Wang
University of Southern Mississippi




More information about the Methods mailing list