Metabolic labeling

T. S. Pillay tpillay at ucsd.edu
Thu Dec 22 14:08:21 EST 1994


In article <userggza.326.000F8FC1 at umich.edu> , userggza at umich.edu writes:
>Does someone know the source of the detailed protocol for metabolic
labeling technique?
>What resin/column (source?) is the best for mobilization/purification of 
>proteins after labeling? Does someone have experience with smooth muscle
cells 
>using this particular technique?

You need to be more specific:  i.e. which isotope are you thinking about
and what's the protein in question?  Most commonly, the protein is
immunoprecipitated after labelling. Try the Methods in Enzymology series.



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