database hit frequencies

Varyc varyc.mmcri at OFFICE.MMC.ORG
Fri Dec 23 11:31:45 EST 1994


Hi netters,
I have several questions regarding the use of the Blast nucleotide alignment algorithm . Any input
would be appreciated.

They are:
1. How does one interpret the scoring data returned with potential matches found for an
anonymoue query sequence using xgenquest/blast

2. If one were to launch 100 anonymous 300-500BP sequences (say from an ABI sequencing
project) derived from a restriction digest (EcoR1, Sau 3a) how many significant "matches" with
known sequences would be expected; would the fraction matching be similar to the fraction of the
genome sequenced? 

Thanks in advance for any help,

Cal Vary



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