nuclei prep

Denize Lepley dmlepley at
Fri Dec 23 16:13:22 EST 1994

 I am looking for a protocol for preparing whole nuclei from
 mouse keratinocytes.  Because keratinocytes contain many
 keratins, preparing whole nuclei from them is very difficult.
 The nuclei will be used to assay a nuclear kinase, so a protocol
 using reagents containing phosphatase inhibitors is desired.  

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