Differential Display of Artefacts?
Shahram Mori
smori at nmsu.edu
Mon Dec 26 18:19:15 EST 1994
Michael Cooley (szcooley at dale.ucdavis.edu) wrote:
: There is a lot of ways for it to fail. What does it look like when its
: not working?
: #%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%
: % #
: # Dr. Michael Cooley Practice Random Kindness %
: % mbcooley at ucdavis.edu and Senseless Acts of Beauty #
: # %
: %#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#
I have been having problems too. I have been trying this in proc systems. I
use random hexamers for the cDNA synthesis portion and 1 10mer for the PCR.
A few times it has worked and many times it hasn't. I don't know why.
Cheers,
--
Shahram Mori _/\_
Program in Molecular Biology _\ /_
Dept. of chemistry and Biochemistry Box 3C \_ _/
NMSU Las Cruces NM ||
88003
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