Eric Daniel Slosberg
eds9 at columbia.edu
Mon Dec 26 14:02:09 EST 1994
Does anyone have experience/knowledge about cloning ss
oligonucleotides into a vector. Do I need to make a complementary oligo
and anneal to make a dslinker? Is it possible to just ligate the oligo
into the vector and fill in the second strand w/klenow? Also what
ratio of insert:vector is needed for this type of cloning (especially if
I make a blunt ended dslinker). Any advice can be posted on this newsgroup.
Thanks in advance.
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