cloning oligos

Eric Daniel Slosberg eds9 at columbia.edu
Mon Dec 26 14:02:09 EST 1994


Hello:
	Does anyone have experience/knowledge about cloning ss 
oligonucleotides into a vector.  Do I need to make a complementary oligo 
and anneal to make a dslinker?  Is it possible to just ligate the oligo 
into the vector and fill in the second strand w/klenow?  Also what 
ratio of insert:vector is needed for this type of cloning (especially if 
I make a blunt ended dslinker). Any advice can be posted on this newsgroup. 
Thanks in advance.





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