srutt at beagle.Colorado.EDU
Mon Dec 26 22:33:57 EST 1994
Eric Daniel Slosberg <eds9 at columbia.edu> writes:
> Does anyone have experience/knowledge about cloning ss
>oligonucleotides into a vector. Do I need to make a complementary oligo
>and anneal to make a dslinker? Is it possible to just ligate the oligo
>into the vector and fill in the second strand w/klenow? Also what
>ratio of insert:vector is needed for this type of cloning (especially if
>I make a blunt ended dslinker). Any advice can be posted on this newsgroup.
>Thanks in advance.
see Derbyshire, et al, Gene 46: 145-152 `86 (ssOligo cloning)
steve srutt at beagle.colorado.edu
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