cloning oligos

[Ben-Sapiens]

Eric Daniel Slosberg <eds9 at columbia.edu> writes:


>Hello:
>	Does anyone have experience/knowledge about cloning ss 
>oligonucleotides into a vector.  Do I need to make a complementary oligo 
>and anneal to make a dslinker?  Is it possible to just ligate the oligo 
>into the vector and fill in the second strand w/klenow?  Also what 
>ratio of insert:vector is needed for this type of cloning (especially if 
>I make a blunt ended dslinker). Any advice can be posted on this newsgroup. 
>Thanks in advance.

see Derbyshire, et al, Gene 46: 145-152 `86    (ssOligo cloning)

steve           srutt at beagle.colorado.edu