Pseudomonas contamination

Kit IO20630 at MAINE.MAINE.EDU
Mon Dec 26 16:10:56 EST 1994


Hi! Can anyone give me a suggestion on how to avoid the
contamination of my YT plates (50ug/ml Ampicillin added)
after I plated the transformants onto them.  I did not have
this problem before but it occurs quite often recently even
though I steriled everything.  I wonder if there is a vector
which carrys an antibiotic gene that can kill or suppress the
growth of Pseudomonas ( I suspect this is the contaminant ).
Thank you.

Kit Tam
UMaine



More information about the Methods mailing list