Mouse liver DNA?
mctoloc at bimcore.emory.edu
Tue Dec 27 19:32:25 EST 1994
In article 2112940900460001 at clinical-mac-90.ucsd.edu, lremington at popmail.ucsd.edu (Lee H. Remington) writes:
>I am trying to make genomic DNA from mouse liver. Unfortunately, after
>prot K digestion of homogenized tissue and phenol/Chloroform
>extraction/ethanol precipitation, I am getting a flocculent white
>precipitate coming out with the DNA. I am not seeing the nice stringy
>genomic DNA in my precipitation that I expect. I have tried multiple
>phenol/chloroform extractions and precipitations but this white junk won't
>disappear. Any idea what this precipitate is? lipoprotein? what can be
>done to get rid of it? Maybe there is a better part of the mouse to make
>DNA from? I need lots for multiple southerns (15 µg/ southern).
>Any input would be greatly appreciated!
It is likely glycogen. Try making the DNA from some other tissue,
otherwise, npo for 24 hours if the animal people will allow it.
Dept of Pharmacology
Emory University School of Medicine
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