cycle vs. normal sequencing

[Karl A. Suiter]

In article <mic-2812941448430001 at 165.124.225.226>, mic at nwu.edu (Mic
Chaudoir) wrote:

> In addition to the comments people have stated before, I would add the
> following remarks:
> 
> Cycle sequencing requirtes the use of P32 (as people have mentioned... you
> can use P33, but it is very pricey).  In addition to the greater rad
> hazard from P32, the sequence is also much less readable than S35, IMHO.
> 
> -- 
> mic
> mic at nwu.edu

Ummm???  We use the Stratagene Exp(1-) pfu Cyclist cycle sequencing kit
and get excellent results with S35 using either a Perkin Elmer or Hybaid
Omnigene thermal cylcler.

Karl

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