Green Fluo. Prot.

David J Askew askewd at gas.uug.arizona.edu
Wed Dec 28 15:11:53 EST 1994


Hello All,
	I'm looking for advice and experience in using the Green Fluorescent
Protein (GFP) marker in combination with FACS analysis.  I'd like to use 
it in mammalian (mouse thymocyte) cells.  The idea is to drive GFP 
expression by a promoter of interest and isolate the subpopulation of 
GFP-positive cells for further study.  Anyway, are people finding the GFP 
marker useful for FACS analysis in mammalian or other tissue culture 
systems?  Positive and negative experiences are greatly appreciated.

Thanks, Dave Askew
	askewd at gas.uug.arizona.edu



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