cla I site in Bluescript II
Rick Neubig
RNeubig at umich.edu
Wed Dec 28 14:18:44 EST 1994
I am interested in using pBluescript II in a PCR mutagenesis
strategy but my cDNA has only a Pst I Cla I fragment as a
convenient insert. I see that the Stratagene map of pBluescript II
has Cla I in parentheses. Does this mean that the cleavage is not
good or that there is another site in the vector for Cla I?
Thanks in advance for the info.
_________________________________________________________
Rick Neubig RNeubig at umich.edu
University of Michigan Phone (313) 763-3650
http://www.umich.edu/~rneubig FAX (313) 763-4450
More information about the Methods
mailing list