cla I site in Bluescript II

Rick Neubig RNeubig at umich.edu
Wed Dec 28 14:18:44 EST 1994


I am interested in using pBluescript II in a PCR mutagenesis 
strategy but my cDNA has only a Pst I Cla I fragment as a 
convenient insert. I see that the Stratagene map of pBluescript II 
has Cla I in parentheses. Does this mean that the cleavage is not 
good or that there is another site in the vector for Cla I?

Thanks in advance for the info.
_________________________________________________________
Rick Neubig                             RNeubig at umich.edu
University of Michigan               Phone (313) 763-3650
http://www.umich.edu/~rneubig        FAX   (313) 763-4450



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