cla I site in Bluescript II
RNeubig at umich.edu
Wed Dec 28 14:18:44 EST 1994
I am interested in using pBluescript II in a PCR mutagenesis
strategy but my cDNA has only a Pst I Cla I fragment as a
convenient insert. I see that the Stratagene map of pBluescript II
has Cla I in parentheses. Does this mean that the cleavage is not
good or that there is another site in the vector for Cla I?
Thanks in advance for the info.
Rick Neubig RNeubig at umich.edu
University of Michigan Phone (313) 763-3650
http://www.umich.edu/~rneubig FAX (313) 763-4450
More information about the Methods