mRNA from Total RNA: Pref Methods?
slawek at ALTIS.CHEM.PG.GDA.PL
Wed Dec 28 12:34:25 EST 1994
Try use streptavidyne beads with biot-oligo T from Promega.
slawek at altis.chem.pg.gda.pl
On Wed, 28 Dec 1994, Michael Myers wrote:
> Two questions:
> 1) Is the b.m.methds-reagnts FAQ still in existence? I checked the mit
> faq archive and found nothing.
> 2) Since I couldn't check the FAQ... Is there any consensus among the
> frequent posters in this group on the preferred method for purifying
> polyA RNA from total? I hope this hasn't been a recent thread, I haven't
> read this group in a long time. My coworkers here don't like oligo-dT
> columns because they are slow and waste sample (due to retained volume).
> I recently used the derivatized mAP paper from Amersham. Since it was
> the first time I ever tried to isolate mRNA, I can't comment on it's
> efficiency. Unfortunately, I discovered last week that Amersham is
> discontinuing this product.
> What are some good alternatives?
> michael myers
> the rockefeller university
> myersm at rockvax.rockefeller.edu
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