"best" polymorphic CA repeat promers

Varyc varyc.mmcri at OFFICE.MMC.ORG
Thu Dec 29 14:18:52 EST 1994


Hi all,
Can anyone suggest one or more pairs of PCR primers that :
1. result in amplification of a very highly polymorphic human genomic locus (>10 alleles?).
2. give allele size differences that are easily relolvable by native (preferred) PAGE
3. Possess better than usual sensitivity ie would detect a small amount of one DNA source
(genotype) against a background of excess second genotype.

Thanks in advance for any help.

Cal Vary



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