RT-PCR

Andre Hamel hamel at cc.umanitoba.ca
Thu Dec 29 13:55:22 EST 1994


I've heard that these Qiagen RNeasy kits are not reliable & that they are 
considered to be a "beta-test" product. 

IMHO, stick with AGPC (acid GITC phenol chloroform) methods, whereby 
cells are lysed in GITC, aliquoted, then stored frozen @ -70*C.

cheers,
                            ********************
  Andre Hamel                      email: hamel at cc.umanitoba.ca
  Manitoba Government                         tel: 204/945-7630
  Department of Agriculture                   FAX: 204/945-8062
  Veterinary Services Branch          
  Winnipeg, Manitoba, CANADA 
			  ********************


>QIAGEN has a total RNA kit you can use to quickly prepare total RNA called
>RNeasy.

>-- 
>enof at kaiwan.com  | The highway's jammed with broken     |    Bruce
>enof at aol.com     | heroes on a last chance power drive  | Springsteen



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