Blunt ended ligation

Duncan Clark Duncan at genesys.demon.co.uk
Sat Dec 31 08:02:11 EST 1994


In article: <3dqfi4$6jl at nermal.cs.uoguelph.ca>  ssharif at uoguelph.ca (Shayan Sharif) writes:

> 5- A range of MgCl2 and ATP final concentrations, from 50 to 100 mM
> and 0.1 to 1 mM respctively.
> 

High ATP ie 1mM specifically inhibits blunt ligations. I remember this 
being used for cosmid ligations when inhibition of blunt ligation but 
ligation of sticky ends was required.

Duncan


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