Blunt ended ligation

Duncan Clark Duncan at
Sat Dec 31 08:02:11 EST 1994

In article: <3dqfi4$6jl at>  ssharif at (Shayan Sharif) writes:

> 5- A range of MgCl2 and ATP final concentrations, from 50 to 100 mM
> and 0.1 to 1 mM respctively.

High ATP ie 1mM specifically inhibits blunt ligations. I remember this 
being used for cosmid ligations when inhibition of blunt ligation but 
ligation of sticky ends was required.


My mind's made up. Don't confuse me with the facts!

More information about the Methods mailing list