Methods December 1994 Archives by date

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Starting: Thu Dec 1 00:09:47 EST 1994
Ending: Sat Dec 31 20:06:24 EST 1994
Messages: 894

Help needed for partial digestion of genomic DNA Kah-Tong, Seow
Help needed for partial digestion of genomic DNA Kah-Tong, Seow
Other GUS substrates? HAUSER MARIE-THERES
Thermostable DNA pol w/o RT activity? Olav Hungnes
Is there any inexpensive way to separate RNA from DNA? Song Tan
How to separate plasmid DNA from Chromosome DNA? Song Tan
P1Tn9clr100 Duncan Clark
vistra/catalyst hl15 at hubrecht.nl
RNA isolation from PANCREAS POPOVA
PRof Jack Strominger, Harvard G Gallagher
Prof Thomas Spies, Harvard G Gallagher
FIAU once again ! Ahnders Franzen
RNA isolation from PANCREAS POPOVA
Plasmid pBMGNeo Jeffrey Upperman
Square Petri dishes about 100 x 15 mm Jacob Peider Hofman-Bang
what is NP-40? Christopher G. Alpha
DNA purification problem: glycogen Antonio Rodriguez-Ariza
Promotors wanted ! Ahnders Franzen
wizard DNA clean up David Barrass
Making electrophoresis boxes dkim at unm.edu
RNA isolation from PANCREAS!!! Bruno Becker
OCT ae at dna.bio.warwick.ac.uk
Where can I get KIRROMYCIN? Torben Osterlund
fax number for osaka bioscience institute wch190 at lulu.acns.nwu.edu
Biotin derivative-coupled dNTP's? Dale Yuzuki's Work Account
Phosphorimager redux T Bannor
FAQ Sasha Kraev
GST-fusion-retinoblastoma Thomas C. Chiles
problems with DIG Sasha Kraev
antibody to murine Cdk4 Thomas C. Chiles
DNase FOOTPRINTING Off a Gel-Retarded Complex Ken Doyle
Plant DNA PCR Sasha Kraev
Other GUS substrates PRAKASH at ACD.TUSK.EDU
On density of DNA Antonio Rodriguez Franco
REPORTER GENE ASSAY/Elisa format BIOTROP at BIOTECHNET.COM
Mobitec Jon Chappel
what is NP-40? Philip Heacock
Source for HA antibody in Europe? Cornelius Krasel
Ras activity Lani Louise Longenecker
Stratagene's Quickhyb solution........ Robert Fisher
FIAU once again ! Klaus.Matthaei at ANU.EDU.AU
Wizard DNA clean up Richard Kenneth Bruce Martel
Requesting Nuc Acids Res Author's Instructions RiverSloth
REPORTER GENE ASSAY/Elisa format Martin Leach
RNA zoo blots for northerns being
fuzzy pcr "PBS::NELSONJ" at PBS.DFO.CA
Genbank comparison Marc Goldstein
Nightmare of Baculovirus expression system Clifford Beall
plasmid vectors for yeast two-hybrid system? bcraft at darwin.bio.uci.edu
Help me on RNA sequencing JongSub Lee
microsatellite help Beth Wapelhorst
fuzzy pcr Beth Wapelhorst
Requesting Nuc Acids Res Author's Instructions Nigel Eastmond
Making electrophoresis boxes lwlkarel at rivm.nl
Phosphorimager question Juha Kononen
Making electrophoresis boxes lwlkarel at rivm.nl
Y-shaped RNA structures help? meine
making electrophoresis boxes Sasha Kraev
dsDNA sequencing protocol Andreas Buhr
Negative staining with Ponceau red GWDGV1::KCHOWDH
David Strehlow's address Dave Gilbert
fuzzy pcr - Reply Varyc
Biotin derivative-coupled dNTP's? - Reply Varyc
MoBiTec Address Sasha Kraev
Address of CRINOS Gary Hellermann
Quantitative RNA Extraction David P Creely
Grad Work Proposal Cynthia D. Furlong
DNase FOOTPRINTING Off a Gel-Retarded Complex Sandy Curtiss
SSCP analysis orbashu at ubvms.cc.buffalo.edu
David Strehlow's address Martin Leach
PCR of formalin fixed tissues slebos at amc.uva.nl
trk promoters Joseph Verdi
hGH expression plasmid wanted. Bill Colledge
PCR: Why is my TAQ unfaithful Zophonias O. Jonsson
Making electrophoresis boxes B.L.Cohen
Mobitec Jon Chappel
use of spermidine to remove DNA from prot. solutions Alberto I Roca
Cheap Thermocycler Mark J. Rieder
FarWestern Analysis Brad Fabbri
milky gels, sequencer hint(?) Robert Preston
Techne Gene E thermocyclers dpetersn at unixg.ubc.ca
Abs for urokinase VBgroup
gum arabic update John True
DNase with activity at alkaline pH? Roger Graham
Wanted: Plant tubulin cDNA for constitutive probe Michael R. Frank
archive/unsubscribe mouse at LAMAR.COLOSTATE.EDU
potato RNA Richard Dana
ds DNA sequencing surefire protocol wanted. friedman_j at mscf.med.upenn.edu
Tricine buffer for PCR Michael Cooley
what is NP-40? Michael Cooley
what is NP-40? Gary Butler
fitc labeling Gary Butler
what is NP-40? Cornelius Krasel
making electrophoresis boxes HARDIES at THORIN.UTHSCSA.EDU
pulsed field gel electrophoresis Tom Zahrt
Blotter-comparison christian a geltinger
Tricine buffer for PCR Frances Hannan Zoology
pulse-field gel ...... POPOVA
Cosmid library, trans-acting factors astlab4
NEED Protein-Protein interaction controls $ Jonathan Marc Rothberg
Help: Warped Polyacrylamide Gels Guy Hoelzer
advice on phosphorimager purchase Tracy Aquilla
Methylation analysis/bisulphite sequencing Jon Nakamoto
Cheap Thermocycler Jon Nakamoto
commercial source for E. coli mutator strain? Dr. Karl Dane Wittrup
aeromonas transformation 190802751 Lawrence, Paul
Help: Warped Polyacrylamide Gels Beth Wapelhorst
beads for transformation Dr Tay Hou Ngee Agnes
what is NP-40? Gary Butler
GenHunter Jim Hutchins
what is NP-40? Jim Hutchins
beads for transformation Dr Tay Hou Ngee Agnes
beads for transformation Dr Tay Hou Ngee Agnes
ArQule Address Robert Nelson
ethidiumbromide lwlkarel at rivm.nl
advice on phosphorimager purchase John Brunstein
sequence of lambda 2001 Tony Harmar
HELP! DIG-labeling by RT KUROKAWA
Converting recA to rec+ Duncan Clark
biotechnology patents PATRICK BRIAN ARBUTHNOT
corn cob bedding for mice? John Dixon
advice on phosphorimager purchase Cornelius Krasel
unsubscribe 0796mhild at UMBSKY.CC.UMB.EDU
Total RNA vs mRNA for diff. display T. S. Pillay
Westerns without blocking step? T. S. Pillay
Plasmid DNA Slawek Dabrowski
Tricine buffers and PCR Gerard T O'Neill
Looking for suppliers of antibodies to Pterins and Pteridines. BSS169 at BANGOR.AC.UK
On density of DNA Michael Cooley
Rather simple ? regarding gel electrophoresis Jim Owens
Converting recA to rec+ Michael Cooley
Total RNA vs mRNA for diff. display Michael Cooley
R Grant Steen Jillian Johnston
unsubscribe mouse at LAMAR.COLOSTATE.EDU
unsubscribe DEPIERI at BCRSAG.AGR.CA
Cheap Thermocycler dkim at unm.edu
Total RNA vs mRNA for diff. display dkim at unm.edu
8 base cutters Mary Remington
Making electrophoresis boxes Paul N Hengen
Want address of Henning Berlin GmbH. BSS169 at BANGOR.AC.UK
test Lana C Stallard
What is spermidine??? Lana C Stallard
Wizard DNA clean up Jeffrey Shane Temple
Allozyme electrophoresis & Crustaceans K.A.Foley
advice on phosphorimager purchase Tracy Aquilla
Returned mail: User unknown Mail Delivery Subsystem
Returned mail: Unable to deliver mail Mail Delivery Subsystem
Biomek routines Callum Bell
clean chloroplastic DNA! Michael Cooley
IMPROPER USE OF METHODS NEWSGROUP!!!!! BIOSCI Administrator
QIAquick Spin Columns Alice L. Hempel
Allozyme electrophoresis & Crustaceans John H McDonald
plasmid vectors for yeast two-hybrid system? Simon E Plyte
glass beads Dr Tay Hou Ngee Agnes
Ditto for the usenet Calvin and Hobbbes
How are you Fikrettin Sahin
Cheap Thermocycler Paul N Hengen
genome size? Michael Cooley
8 base cutters Yasuhiro NAITO
sdfg Jeffrey bronchick
Bead Beater Dale Yuzuki's Work Account
sigma and reagents Jeffrey bronchick
Open Question--has anyone noticed how tight budgets are? Jeffrey bronchick
unsubscribe mouse at LAMAR.COLOSTATE.EDU
Nasilsin faruk
PCR of semen samples Dieter Palmer
On density of DNA Scott B. Mulrooney
PCR primers of c-Jun and c-Fos Hiroyasu Inada
Screening an expression library using liquid lysis of bacteria Neal Gliksman
Quantitative RNA Extraction CJF 90/10 INSERM
Aminobenzamidine N Benson
DNA from old blood samples?? Chris Troy
DNA from old blood samples?? Tim Hutchin
(none) Slawek Dabrowski
The price of biotin has increased 50X Dave Gilbert
Westerns without blocking step? Gary Butler
colocalisation of proteins with ribosomes J.Luirink
what is the best nylon membrane? sl16s at cc.usu.edu
Help with Lambda DNA isolation K.W. Cheung
Problem with non-radioactive hybridization to GC-rich DNA Steven Goldberg
PCR primers of c-Jun and c-Fos Sean David Moore
Problem with non-radioactive hybridization to GC-rich DNA--Repeat Steven Goldberg
Total RNA v mRNA for differential display pattonaj%phvax.dnet at sb.com
Sexing of Birds Martin Leach
WANTED: anti gamma glutamyl carboxyl group Willy Flegel
Human mitochondrial RNA Pol? Mike Morris
commercial source for E. coli mutator strain? Roger Graham
Preventing Precipitation of G Phosphoramidites Wade W Turner
8 base cutters Stuart Kuhstoss
Polite Unsubscribing from methds-reagnts Paul N Hengen
Lambda DASH DNA isolation Sasha Kraev
plasmid sub-genomic libraries Wayne Gottlieb
Primer stocks that fail to PCR over time Paul N Hengen
X-gluc user unknown
PCR of semen samples POPOVA
Open Question--has anyone noticed how tight budgets are? Richard R. Hardy
Open Question--has anyone noticed how tight budgets are? Rae Nishi
HELP: HELICOBACTER PYLORI ASSAY Douglas Ormrod
genius lina bueno
beads for transformation Cornelius Krasel
Yellow Phosphor Imaging Plate George Clark
Multi primers PCR mmai at kean.ucs.mun.ca
Blotter-comparison Stephen R. Lasky, Ph.D.
test gjenkins at STUDENTS.WISC.EDU
equilibrium dialysis rogers elizabeth
DNase FOOTPRINTING Off a Gel-Retarded Complex Ken Doyle
request help with immunofluorescence! BARNETT.YA
DIG-PCR ELISA kit Sandhya Tewari
sperm PCR Jeff Kramer
Sexing of Birds NICHOLAS THEODORAKIS
Is there a cheaper alternative to IPTG? PANIAGUA SOLIS JORGE FERNANDO
RAPD fingerprinting Katherine Nesbitt
Nuclei from Arabidopsis suspension culture kloska at mpimg-berlin-dahlem.mpg.de
looking for Sandie Baldauf ANNE MARIE MURPHY
Is there a cheaper alternative to IPTG? Roland J Saldanha
Help me on RNA sequencing Joan Rowe
Help me on RNA sequencing Shula Zhang
Selective Media Blues... G.Dellaire at PO-Box.McGill.CA
SDS-PAGE gel problem Kitty Kit Chui
half life of GUS, luciferase and other reporters? SRKain
O.C.T.? Jukka Luoma
Multi primers PCR Paul A. Vescio
8 base cutters Anderson Lab
Westerns without blocking step? H.J. Treumann Biochemistry ext 4301
What is the most economical phosphorimager? Dave Gilbert
Test: Please ignore... N.C.Pancholi at reading.ac.uk
8 base cutters Duncan Clark
S&S RAD-FREE kit Graham Atherton
Question about Mulfold (M. Zuker et al.'s) Erik Forbes Y. Hom '95
genome size? Glen Shearer
Protein and agarose gels James J. Campanella
parrot sexing NISSON at BIOTECHNET.COM
PVP: which MW to use? DNA Minipreps... Christopher G. Alpha
Is there a cheaper alternative to IPTG? Tracy Aquilla
Help---GET RID OF POLYSACCHRIDE IN RNA OR DNA EXTRACTION IN PLANT CALLUS Xin-Hua Zhou
Multi primers PCR anon
in vitro Transcription/Translation Sam Volchenboum
EMCV5'NTR Ben Yang
Methylation analysis/bisulphite sequencing Karl Voss
Methylation analysis/bisulphite sequencing Karl Voss
COS7 cells/Triton lysis Lee A. Witters
Native gel problems JMORELLI at MAINE.MAINE.EDU
Selective Media Blues... NICHOLAS THEODORAKIS
Help with Lambda DNA isolation RiverSloth
multiple primer PCR Jeff Kramer
Help, Oligos Fast Carla Drebing
Total RNA vs mRNA for diff. display Mike Rott
SDS-PAGE gel problem skoufos at nefeli.imbb.forth.gr
what is NP-40? Uwe Harmening
Controlling Hybaid OMNIGENE with a PC/Mac Charlie Wright Genetics
Separation of DNA from glycogen? neale at mbcf.stjude.org
f-met tRNA rogers elizabeth
Help with Lambda DNA isolation Jean L Dowty
Baculovirus/Pichia:Tunicamycin inhibition of N-glycosylation Ned Lally
What is spermidine??? Michael D Kawalek
colocalisation of proteins with ribosomes Richard_Heath
Silastic Tubing Jack Lancaster
Need help for emoving glycogen from DNA solution Uttam Rajbhandary
Q: searching for phosphorylation sites Erik Forbes Y. Hom '95
Multi primers PCR Bob Harrison
8 base cutters Martin Kennedy
Wish to tag protein with SV40 T Ag NLS cotto at casbah
Help looking for Bax antibody Kristine Lee
H.pylori elisa nswbch09 at angis.su.oz.au
bcl-2 molecular mass ADRIAN JORDAAN PUREN
PCR for mycoplasma mazella at naxos.unice.fr
tRNAnscend(TM) Christopher Coward Path
Multi primers PCR Karel Wernars
DNAscan software from MWG? Pierre Saumitou
lambda-vector? Daniel Schlieper
milky gels, sequencer hint(?) Song Tan
EMCV5'NTR GWDGV1::KCHOWDH
Is there a cheaper alternative to IPTG? Song Tan
Sexing of Birds schupp at nauvax.ucc.nau.edu
SSCP detection schupp at nauvax.ucc.nau.edu
Help with Lambda DNA isolation Peter Emanuel
Drying sequencing gels orbashu at ubvms.cc.buffalo.edu
PCR for mycoplasma G Gallagher
Primer selection Tim Hutchin
What is the most economical phosphorimager? Tracy Aquilla
Westerns without blocking step? Tracy Aquilla
Large scale P1 prep Dan Burgess
Boeh.Mannhiem Cell. DNA Frag. ELISA Mary Remington
RNA isolation from PANCREAS!!! krzych
SSCP detection HARDIES at THORIN.UTHSCSA.EDU
RACE-PCR tdstrugn at acs.ucalgary.ca
artifact in stratagene lambda zap libraries
HELP WITH TRANSFORM./COMP.CELLS!!! Darren Stauth
PCR for mycoplasma CURTIS SUTTLE
cDNA Wenjun Huang
PCR replication slippage Laura Saward
Primer selection Michael Gorry
Request for info: mouse bcl-2 and bax DNA probs C. McCulloch
Isolation of Nuclei In C. Kim
what is NP-40? john markwell
Drying sequencing gels Richard Kenneth Bruce Martel
What is comparative EST? Dale Yuzuki's Work Account
Multi primers PCR Nancy Rawson
Wizard Preps: DNA Doesn't Freeze Guy Oshiro
Drying sequencing gels Colin Rasmussen
Drying sequencing gels DNA Rules!
Total RNA vs mRNA for diff. display Michael R. Frank
RT reagents affect PCR Michael R. Frank
Selective Media Blues... Jon Nakamoto
RT reagents affect PCR Steve Hopkins
Gene Construction Kit sdj002 at acad.drake.edu
PCR for mycoplasma Alan Lyons
Transcriptionally active nuclei from Adipose tissue hAPPy gUY
DNA Sequence analysis shareware hAPPy gUY
Drying sequencing gels Steven Goldberg
DNA Sequence analysis shareware Don Gilbert
test please ignore mayaux-bisance
Primer selection David R. Rockhold
2:30pm (GMT) need urgent help CJF 90/10 INSERM
RNA from Qiagen kit Rachelle Leger
universal eukaryote 18S-rDNA primers M O L B I O L
Is there a cheaper alternative to IPTG? Zophonias O. Jonsson
Drying sequencing gels Michael Romero
cDNA Thomas Newman
what are Saponins used for? Michael Levin
Codon Usage Emma Macfarlane
Source for HA antibody in Europe? Gary Rudnick
glass beads Tom Chappell
ssDNA templates by i primer PCR Gareth
Cell wall degradation desired.. Castor Menendez
pGEM-T woes Rae Nishi
Reverse transcription and RNA second. structure krzych
primer selection Kirk A. Bartholomew
Mulfold Zeiler, Brian M'bio
Peptide Acceptors for N-linked glycosylation Randall Howard
HELP WITH TRANSFORM./COMP.CELLS!!! Zhixiang Hu
Drying sequencing gels Stephen R. Lasky, Ph.D.
On commercial protein sequencing Antonio R. Franco
Silastic Tubing Gary Radice Radice at urvax.urich.edu
Wizard Preps: DNA Doesn't Freeze Stephen R. Lasky, Ph.D.
baculovirus vector ? ffr.de
what are Saponins used for? Kamin Johnson
Reusing Qiagen columns gwhatfield
HELP WITH TRANSFORM./COMP.CELLS!!! bipin dalmia
Help with Lambda DNA isolation Amos H Heckendorf
Lambda Sorb kertesz at micro.biol.ethz.ch
Help looking for Bax antibody Steven L. Sabol
Reverse transcription and RNA second. structure Stephen R. Lasky, Ph.D.
Where Can I Buy A Hybridization Oven? Barry Moore
Cell wall degradation desired.. David R. Rockhold
pCMV4 sequences needed gsbs1018 at UTSPH.SPH.UTH.TMC.EDU
baculovirus vector ? Brad Romney
To DNA sequencing experts! JongSub Lee
PCRing B-gal transgene? Jennifer Schmitt
RNA from Qiagen kit Yasuhiro NAITO
Native gel problems Jim Hutchins
Is there a cheaper alternative to IPTG? John Altman
Is there a cheaper alternative to IPTG? Song Tan
glass beads for plating bacteria to get an even distribution on agar Pete Muriana
Autofluorescence cures? Corrie Allen
Mutagenesis Kits, advice Chuck Hauser
biotin-lys-tRNA Karl Marx Mac
Anti CD9 mAB BA6 George W Chacko
Native gel problems Dietmar Tietz
Source of B16 melanoma cells? Shane Albright
Wish to tag protein with SV40 T Ag NLS Tom Thatcher
genome size? Andrew Cockburn
On density of DNA Andrew Cockburn
Wizard Preps: DNA Doesn't Freeze rhubner at molbiol.ox.ac.uk
Does anyone have antibody or cDNA for ICE? James
373 Stretch (PE/ABI): Sequenase Kits G. Dorado
Sequencing PCR-amplified human hprt cDNA with 373 Stretch G. Dorado
problems with DIG G. Dorado
cloning vector for 23kb Mr. C.S.Wilding
DIL staining SPAGNOL at PLAUTO.CSATA.IT
HPLC capability question. BSS169 at BANGOR.AC.UK
MJResearch?? Mark J. Rieder
MJResearch?? Colin Rasmussen
cleaning PCR primers Biochemistry Lab computer
T-cloning vs. Srf-cloning? kvernick at helix.nih.gov
to DNA sequencing experts Sasha Kraev
cleaning PCR primers,correction Jo Caine
Source of B16 melanoma cells? Shane Albright
unsubscribe SCHALLER%CFRUNI51.earn at earn-relay.ac.uk
journal for antisense DNA Piroska Rakoczy
(none) Slawek Dabrowski
DNA BIOCHEMISTRY TEXT (fwd) Philip OBrien
Wizard Preps: DNA Doesn't Freeze Christopher Coward Path
Protein and agarose gels Christopher Blencowe
Aktieselskabet Grindstedtvaerket address ? U196CFA at CSI.IT
HELP WITH TRANSFORM./COMP.CELLS!!! Duncan Clark
potato RNA Sean David Moore
HELP WITH TRANSFORM./COMP.CELLS!!! Rachelle Leger
Yellow Phosphor Imaging Plate Sean David Moore
RT reagents affect PCR Sean David Moore
competent cells and ampicillin Sasha Kraev
Drying sequencing gels Annette C. Hollmann
cleaning PCR primers David R. Rockhold
Thermometer question kmorris1 at opal.tufts.edu
Transcritional Start Site klingj
rep PCR for bacteria/MLO's Marcella E Grebus
cleaning PCR primers HARDIES at THORIN.UTHSCSA.EDU
Cleaning PCR Primers schoenfeld at MSSCC.MED.UTAH.EDU
primer selection HARDIES at THORIN.UTHSCSA.EDU
question about yeast GAL4 DNA binding sequence PRAMATHESH PATEL
Thermometer question Martin Leach
cleaning PCR primers Steve Hopkins
cleaning PCR primers,correction Steve Hopkins
Thermometer question K. Legate
Yellow Phosphor Imaging Plate venita de almeida
Protein expression MUNGO at UVVM.UVIC.CA
Co. phone--BioFluids Virginia Dress
Thermometer question Colin Rasmussen
reuse of agarose Michael Cooley
Help with sequencing Geoff Goellner
sending bugs and plasmids? Dr Tay Hou Ngee Agnes
protein expression problem (maybe SS bonds) HARDIES at THORIN.UTHSCSA.EDU
Ditto for the usenet bcdlee at hkpcc.hkp.hk
histone and human estrogen receptor probes bcdlee at hkpcc.hkp.hk
PROBLEM RESOLVING H & L chain intermediates on gel Oh Kah Weng Steve
Autofluorescence cures? dhanzel at delphi.com
pGEM-T woes Timo Hiltunen
Reverse transcription and RNA second. structure Terry Hanzlik
Osteocalcin promotor wanted ! Ahnders Franzen
Generalized Transcription Inducers ?? Ng Hian Cheong
preparation of CsTFA solution?
animal software Josef Peter Magyar
Chymotrypsin: Optimal buffer conditions? Mathias Bergman
Help with Lambda DNA isolation Allan Hayle
enhancerless promotors Varyc
Lawrist4 vector sequence Mr. R.J. Stephens
Osteocalcin promotor wanted ! Stephen R. Lasky, Ph.D.
pJ3 vector sequence? Patrick Grealish
journal for antisense DNA Shaun D. Black
concentrating protein lysates Mary Remington
Looking for lambda-vector with NotI-site(s) Joerg Suckow
editing problem with large constructs converrl at ucbeh.san.uc.edu
Help! Need vector w/out homol. to pBR322 ljw at rpiv.mc.duke.edu
Terminator sequencing reactions with Sequitherm? Muhammad Lodhi
HELP! Method for sequencing 5'ends of cDNA ("RACE") Edwin Fink
Panning of Phage display Library P. Emanuel
Lawrist4 vector sequence Frances Hannan Zoology
Looking for: Method for sequencing 5prime ends of cDNA ("RACE") Hr. fink clinbio
concentrating protein lysates Stephen R. Lasky, Ph.D.
glass beads for plating bacteria to get an even distribution on agar Anthony C.
Strepavidin Beads Karl Voss
concentrating protein lysates Colin Rasmussen
ligation-mediated PCR Tom Doak
Transcritional Start Site Traci Roth
test KUMAR
uniform colony distribution Robert Preston
(none) Bruce J. Turner
cheap sequencing setup? Steven Hecht
wizard DNA cleanup See.my.signature
Metabolic labeling userggza at umich.edu
Storage of purified proteins Gene Holowachuk
RNA isolation from PANCREAS Richard Schifreen
rep PCR for bacteria/MLO's Robert Preston
baculovirus vector ? bipin dalmia
Protein expression bipin dalmia
TriZOL reagent for RNA isolation? Richard R. Hardy
kodak x-ray film sensitivity john hachey
wizard columns Bruce J. Turner
Help: need a model for prokaryotic 16S rRNA vivek kapur
RT of tRNA Peter Gegenheimer
Labeling with PCR Bruce Elder
Is there a cheaper alternative to IPTG? Michael Galperin
Sau96I doesn't cut well Claudia Benkwitz
RT reagents affect PCR Loryn Sellner
artifact in stratagene lambda zap libraries Rob Steele
Labeling with PCR Colin Rasmussen
Help with Nylon Membranes Ed Madison
rep PCR for bacteria/MLO's lwlkarel at rivm.nl
pGEM-T woes Gerard T O'Neill
STILL LOOKING FOR KIRROMYCIN Bjoern Voldborg
GEM-T woes Sasha Kraev
Size fractionation and genomic library KUMAR
Protein dimerization detection protocol wanted please Ibelgaufts at vms.biochem.mpg.de
Test KUMAR
RNA folklore? - Reply Varyc
Size fractionation and genomic Library KUMAR
help with (cycle) sequencing Sasha Kraev
STILL LOOKING FOR KIRROMYCIN Edwin ten Dam
STILL LOOKING FOR KIRROMYCIN Brad Riesenberger
Guinea pig liver cDNA library Ken Minneman
pJ3 vector sequence? Jim Woodgett
help with seq. (why end label for cycle seq.) HARDIES at THORIN.UTHSCSA.EDU
S.O.S. PROTEIN OVEREXPRESSION!!!!! Jorge.Hernandez at bota.unine.ch
RNA folklore? hrasal at marc.cri.nz
yields on low melt? Emanuel
Labeling with PCR schoenfeld at MSSCC.MED.UTAH.EDU
preparation of CsTFA solution? Lauri Lintott
Automated Sequencer Erdmann Dirk MSM PH225
Storage of purified proteins Richard_Heath
RNA folklore? NICHOLAS THEODORAKIS
journal for antisense DNA Jack M. Bernstein, M.D.
pVE6060 tn delivery vector DAB
Dissolving Qiagen purified DNA Dave Bates
T7 DNA pol for SD mutagenesis Karl Fischer
GUS staining user unknown
Transcritional Start Site a-library
DNA types and sources Gabriel Akopian
HELP WITH TRANSFORM./COMP.CELLS!!! William B. Melchior, Jr.
For Sale: HP MS Engine John Brookes
Thermometer question William B. Melchior, Jr.
****Program(S) for antigenic sites???****** Hirdeypal S. Bhathal
Storage of purified proteins Curt Ashendel
Dissolving Qiagen purified DNA Martin Leach
companies AJESURUM at OPAL.TUFTS.EDU
Macvector purchase? Lynn Stam
T7 DNA pol for SD mutagenesis Chuck Hauser
On density of DNA Paul N Hengen
uniform colony distribution Paul N Hengen
RNA folklore? Curt Ashendel
Ox. reagent for proteins lw75
Strepavidin Beads Paul N Hengen
RNA folklore? Cornelius Krasel
synthetic RNA stability/degradation robynb
Lactococcus lactis peptidoglycane Lothar Steidler
BeadBeater vs. The Sonicator Loren Joseph
A source for PALYTOXIN carlos h. pedemonte
Glycerol vs. Glucose in bacterial media Loren Joseph
labelling with PCR Zal Suldan
concentrating protein lysates John A. Newitt
pGEM-T woes Rae Nishi
Storage of purified proteins John A. Newitt
Molecular cloning Phandaal
DIG with whole blood Surasakdi Wongratanacheewin
SAP degrades my vector James Gray
Tricine buffer for PCR
Protein Storage Seth M Fisher
Anti Luciferase antibodies ? chevalie-bisance
pCMV5 sequence wanted k.d.preuss
BeadBeater vs. The Sonicator chelack at admin3
Cellulose-acetate Electrophoresis Andreas Langsdorf
SAP eats my vector Sasha Kraev
seq problems hauge at Frodo.MGH.Harvard.EDU
cheap sequencing setup? Cornelius Krasel
Ox. reagent for proteins Cornelius Krasel
Dissolving Qiagen purified DNA Carsten E. Stidsen
Putting biotin on primers Marc J. Mass
Anti Luciferase antibodies ? Martin Leach
Ox. reagent for proteins Robert Solomon Bioc
Transcritional Start Site klingj
Southwestern ref? Louis Hom
Screening for persence of restriction system Tom Warnick
editing problem with large constructs Stuart Kuhstoss
Glycerol vs. Glucose in bacterial media Robert Solomon Bioc
sending bugs and plasmids? Richard_Heath
Seq problem Sasha Kraev
seq problem/correction Sasha Kraev
Help - Looking for Best Method Charles Ott DuPont - NEN Research Products
Canadian Researchers Ian Clarke
synthetic RNA stability/degradation NICHOLAS THEODORAKIS
FREE PCR Primer Design Software Aaron Apte
Help with cleaning up in vitro transcripts Paul E. Ulanch
Seq. gels ibbl100 at indyvax.iupui.edu
Ras activity assay Lani Louise Longenecker
job offer - molbio reagent sales specialist, Canada Peter Burke
cloning vector for 23kb ibbl100 at indyvax.iupui.edu
Automated Sequencer Lawrence Washington
ACTUAL Restr.-enzyme database wanted... Peter Rudolph
TOC P. Knygsman
Tricine buffer for PCR Jim Owens
Glycerol vs. Glucose in bacterial media Jim Owens
SAP degrades my vector Daniel Mytelka
Transcritional Start Site Randy Haun
Competent DH10b/p3's? T. J. Murphy
Sequencing question*********:) Shahram Mori
E-mail not reaching you from BIOSCI BIOSCI-REQUEST at NET.BIO.NET
PCR yield Jeff Kramer
Serious Problem with one of your users Dave Mack
TriZOL reagent for RNA isolation? Michael Kloth
Binding DNA templates to agarose beads for invitro transcription warwick at staff.monash.edu.au
Advanced Bacterial Genetics course Stanley Maloy
E-mail not reaching you from BIOSCI BIOSCI Administrator
Ox. reagent for proteins Scott B. Mulrooney
Tricine buffer for PCR Jasper Saris
WARNING - Promega T4 DNA polymerase Malcolm White
Q?: high resolution PAGE GUIVARC_H Dominic
Nucleic Acid Based Technologies Meeting Cambridge Healthtech Institute
TOC P. Knygsman
question about enterokinase Sasha Kraev
one gene, two promoters seppen at amc.uva.nl
Ox. reagent for proteins bipin dalmia
Screening for persence of restriction system Duncan Clark
Glycerol vs. Glucose in bacterial media Duncan Clark
Storage of purified proteins Andreas Buhr
REP elements in E. coli Richard_Heath
companies Richard Schifreen
Glycerol vs. Glucose in bacterial media Gary Yellen
Ambion Technical Service Ambion Technical Service
Correction for the info about a Glyko's kit Ding Ming
To DNA sequencing experts! Mic Chaudoir
Labeling with PCR Mic Chaudoir
Sma/Xma problem-likely answer Dr. Pamela Norton
pcr products "PBS::NELSONJ" at PBS.DFO.CA
journal for antisense DNA Randall J. Cohrs
RT reagents affect PCR Steve Hopkins
HELP! Method for sequencing 5'ends of cDNA ("RACE") Joy L Frestedt
****Program(S) for antigenic sites???****** Carl Lawyer
one gene, two promoters ANDREI POPOV
Chemiluminescence for receptor studies? Natalie Williams
Ribonuclease Protection Assay Controls ? Jeffrey Joseph Martino
Affinity co-electrophoresis KUAN CHEE MUN
pcr products MIcha Ron
Microdissection of yeast spores M. Charbonneau
Ribonuclease Protection Assay Controls ? Martin Leach
Problem with PCR Varada P. Rao Memorial Univ. of Newfoundland St. John's Canada
PCR primers auction BCRUBIN at weizmann.weizmann.ac.il
cross-species PCR Carl Lawyer
Looking for: Method for sequencing 5prime ends of cDNA ("RACE") Eric C. Anderson
Hints for PCR product Autosequencing (ABI) Eric C. Anderson
bir ses ver Serdar Coskun
test, please ignore!
Overlapping Eucaryotic genes MBDMITRY at CCVAX.SINICA.EDU.TW
In situ PCR - Perkin-Elmer Jim Cummins
Video Tapes: Cold Spring Harbor Lab -- Transgenic & ES Cells merlin
luciferase culture plates Steve McKinney
journal for antisense DNA David Ish-Horowicz
West. Blot 125 Iodine and saran wrap Mary Remington
Ribonuclease Protection Assay Controls ? Tracy Aquilla
Cellulose-acetate Electrophoresis Hector Cruz-Lopez
Competent DH10b/p3's? paolo de marco
Canon UK (optics) LOGAND
cross-species PCR Marianna Max
Overlapping Eucaryotic genes Tracy Aquilla
Storage of purified proteins Hiesberger Thomas
Subriden phone# Ms. Zhiping Gu; BIOL (GRAD)
luciferase culture plates schupp at nauvax.ucc.nau.edu
sexing of birds schupp at nauvax.ucc.nau.edu
Help with cleaning up in vitro transcripts ****
Overlapping Eucaryotic genes Dr. YL Du
test, ignore Sunil Shaw
test, ignore Sunil Shaw
PCR A/T CLONING VECTOR gsbs1018 at UTSPH.SPH.UTH.TMC.EDU
Protocol for Metallothionine promoter induction needed Mary Remington
heterologous hybridisation (some principles) HARDIES at THORIN.UTHSCSA.EDU
HELP! Extraction of periplasmic proteins. Alexander V Kravchuk
detecting synaptic proteins Fiberman
PCR A/T CLONING VECTOR Marc Van de Craen
Pedigree software for the mac Josef Peter Magyar
precise quantificatin of genomic DNA from different sources Brian C. Freeman
HETEROLOGOUS HYBRIDISATION PROTOCOL marcvdc at lmb1.rug.ac.be
PCR A/T CLONING VECTOR marcvdc at lmb1.rug.ac.be
Overlapping Eucaryotic genes Stephen R. Lasky, Ph.D.
Automated Sequencer Peter Gegenheimer
need T4 bacteriophage - HELP jim at LUCY.TLI.ORG
RNA folklore? Peter Gegenheimer
Tricine buffer for PCR Peter Gegenheimer
Public Domain Software for the ABI373 Andrew Dizon
To DNA sequencing experts! Michael Cooley
PCR A/T CLONING VECTOR Michael Cooley
precise quantificatin of genomic DNA from different sources Michael Cooley
West. Blot 125 Iodine and saran wrap Klaus.Matthaei at ANU.EDU.AU
PCR trouble-shooting book?? Farid John Ghadessy
PCT A/T cloning vector Sasha Kraev
cross-species PCR Peter Kaub
Silver Sequence DNA Slawek Dabrowski
Human Mit. RNA pol - still looking! Mike Morris
Maxipep "problem" seppen at amc.uva.nl
Inosine in PCR primers Chrystala Constantinidou
To DNA sequencing experts! Mr V. Schoenfeld DOM
Site directed mutagenesis kit Thomas C. Chiles
Pedigree software for the mac Tom Thatcher
HELP! Extraction of periplasmic proteins. schupp at nauvax.ucc.nau.edu
Maxipep "problem" Martin Leach
precise quantificatin of genomic DNA from different sources schupp at nauvax.ucc.nau.edu
precise quantification of genomic DNA HARDIES at THORIN.UTHSCSA.EDU
PCR A/T CLONING VECTOR David R. Nelson
Site directed mutagenesis kit venita de almeida
transgenic mice Gene Y. Yang
Promega challenges LaRoche Taq polymerase patent PromegaCC
Ganciclovir Ahnders Franzen
mdm2 Reto Hartmann
Synthetic Peptide Antibodies John D. Valentich
Transcriptionally active nuclei from Adipose tissue Dr. D.G. Fernig
OCLASSEN Ahnders Franzen
transgenic mice drowens at umich.edu
2-D gel analysis Micheal McCann
Promega challenges LaRoche Taq polymerase patent Martin Leach
Ganciclovir korr. Ahnders Franzen
cleaning PCR primers Steven Goldberg
ligation-mediated PCR John Watson
B cell library for LexA two-hybrid system Sandy Denslow
(none) John E. Tysko
Hints for PCR product Autosequencing (ABI) Guy Hoelzer
HELP! Extraction of periplasmic proteins. Cornelius Krasel
Quinpirole Stability GUIVARC_H Dominic
request hi eff transient transfection system Sunil Shaw
Sequencing question*********:) Daniel Michael Lasser
request hi eff transient transfection system Sunil Shaw
PCR A/T CLONING VECTOR Richard T. Timmer
Hints for PCR product Autosequencing (ABI) J. David Spafford
Promega challenges LaRoche Taq polymerase patent Richard Schifreen
Hints for PCR product Autosequencing (ABI) Luc Simon
PCR trouble-shooting book?? Azlinda Bte Anwar
toxic protein in E.coli Tan Nguan Soon
Overlapping Eucaryotic genes -Reply David Osterbur
toxic protein in E.coli Tan Nguan Soon
toxic protein in E.coli Tan Nguan Soon
toxic protein in E.coli Tan Nguan Soon
PCR trouble-shooting book?? Azlinda Bte Anwar
enzyme storage wangyq at BEPC2.IHEP.AC.CN
Mouse liver DNA? Lee H. Remington
Genetic Screening Meeting Cambridge Healthtech Institute
Infectious Disease Diagnosis Meeting Cambridge Healthtech Institute
Synthetic Peptide Antibodies Rick Neubig
optimization of PCR
puting biotin on oligios NEAL MUSTO
Synthetic Peptide Antibodies John E. Fox
pCMV5 sequence wanted k.d.preuss
cycle vs. normal sequencing Clemens Suter-Crazzolara
Seeking primate DNA's Moens William
affinity purification Danny Altschuler
site-directed mutagenesis and PCR Gerri Newfry
PCR A/T CLONING VECTOR Simon Dawson
screening with a riboprobe Xavier Sarda
PCR A/T CLONING VECTOR Mic Chaudoir
Phospho-tyrosine cleavage Nadesalingam N Palaniyar
Phospho-tyrosine cleavage Nadesalingam N Palaniyar
PCR A/T CLONING VECTOR Randy Haun
Promega challenges LaRoche Taq polymerase patent Martin Leach
Quinpirole stability GUIVARC_H Dominic
protein and RNA purification Fiberman
L. lactis cell wall proteins lothar at lmb1.rug.ac.be
Promega challenges LaRoche Taq polymerase patent Richard Schifreen
toxic protein in E.coli Tan Nguan Soon
Help with tubulin purification Megan Oxberry
cycle sequencing Piroska Rakoczy
Clostridium difficile Rod Putland
Greeting wangyq at BEPC2.IHEP.AC.CN
Promega challenges LaRoche Taq polymerase patent Duncan Clark
Storage of purified proteins Giovanni Maga
Ganciclovir Giovanni Maga
Promega challenges LaRoche Taq polymerase patent Duncan Clark
expression in eukaryonts Siggi Engelbrecht
protein and RNA purification Stephen R. Lasky, Ph.D.
Problems with Kunkel mutagenesis Anthony J. Persechini
List of neato keen home pages Jim Hutchins
Promega challenges LaRoche Taq polymerase patent PromegaCC
Invitation to all netters... :) Martin Leach
List of neato keen home pages Martin Leach
cycle vs. normal sequencing HARDIES at THORIN.UTHSCSA.EDU
UK: type 16 Beckman Rotor Wanted Hani Gabra
more.....serious ones ;) Martin Leach
Protein A : antibody conjugation Udo Reischl
Pcr fragments labelling thierry berthe
Promega challenges LaRoche Taq polymerase patent Pete Muriana
rRNA Thomas Loeffler
PCR A/T CLONING VECTOR Michael Cooley
cycle vs normal sequencing Sasha Kraev
toxic protein in E.coli Colin Rasmussen
RFI: UV crosslinking devices: Likes/Dislikes? Viraj A. Master
Cancer Prognosis Markers Meeting Cambridge Healthtech Institute
riboprobes with biotin Morag Lancaster
Herb medicine study lab? gsbs1018 at UTSPH.SPH.UTH.TMC.EDU
Metabolic labeling T. S. Pillay
Does this strain exist? benedik at uh.edu
gene expression/construction Institute of Gas Technology
Help with tubulin purification Gerri Newfry
RT-PCR Reid, Thomas M.
Problem with PCR Shahram Mori
Temperature sensitive mutants ZOOMmmm
Help with tubulin purification Virginia Dress
Invitation to all netters... :) Virginia Dress
protein expression in E.coli Tan Nguan Soon
PCR A/T CLONING VECTOR kitchingman at mbcf.stjude.org
HRP storage, SPDP and TMB suppliers. BSS169 at BANGOR.AC.UK
Help with Nylon Membranes SHO1
RFI: UV crosslinking devices: Likes/Dislikes? SHO1
Differential Display of Artefacts? SHO1
database hit frequencies Varyc
Pcr fragments labelling Shiao Y. Wang
Protein gel for high MW protein M Y Chen
nuclei prep Denize Lepley
Line1 clone Dan Wells
Sequencing question*********:) Shahram Mori
optimization of PCR Shahram Mori
Phospho-tyrosine cleavage Shahram Mori
rRNA Shahram Mori
RT-PCR Shahram Mori
help Tetsuya Nakamura
DNA polymorphisms and mutations GR Taylor
Protein gel for high MW protein J. Aphale
precise quantificatin of genomic DNA from different sources Shahram Mori
Radioactive probes by PCR Ella
cycle vs. normal sequencing GR Taylor
enzyme storage MAHARAJAH
Radioactive probes by PCR Anita Gould
Drosophila nuclei prep ORSULIC at UNCVX1.OIT.UNC.EDU
Electroporation or Beakonization??? Sunee Kertbundit - ST
Mouse liver DNA? jbt
Differential Display of Artefacts? Michael Cooley
cloning oligos Eric Daniel Slosberg
Radioactive probes by PCR Bert Gold
Pseudomonas contamination Kit
Differential Display of Artefacts? Shahram Mori
rRNA Andrew Cockburn
Ganciclovir Eric Daniel Slosberg
cloning oligos Ben-Sapiens
rRNA Richard Kenneth Bruce Martel
1069 Mr Eric Chan
Private Label Agarose Mr Eric Chan
rRNA Bart.Nelissen
Differential Display of Artefacts? SHO1
RT-PCR Rebecca Schall
Differential Display of Artefacts? Stephen R. Lasky, Ph.D.
enzyme storage Stephen R. Lasky, Ph.D.
HELP!sequencing Elena Baena Gonzalez
PCR Mutagenesis Rick Neubig
cloning oligos Bernard Heymann
Pseudomonas contamination HARDIES at THORIN.UTHSCSA.EDU
HELP!sequencing HARDIES at THORIN.UTHSCSA.EDU
Differential Display of Artefacts? Michael Cooley
Pseudomonas contamination Michael Cooley
sonication lw75
HELP: glutaraldehyde cross-linking I-Jen Huang
Looking for Vectors for chicken muscle cells Qunfeng Dong
sonication J. Tudor
Agarose gel fluorography aris at icbr.ifas.ufl.edu
RT-PCR enof
plasmid prep kits: good & bad? Jon Nakamoto
Clostridium difficile spores Rod Putland
Public Domain Software for the ABI373 Peter J. Neame
Inosine in PCR primers Peter J. Neame
Preventing Precipitation of G Phosphoramid Marie Toloczko
Public Domain Software for the ABI373 Howard Cash
Mouse liver DNA? Marie Toloczko
Blunt ended ligation Shayan Sharif
Source for Oligo(dT)30 latex? Thomas Newman
Almond-N & Kitchin-PA's Email address? Lin Xiao
lambda 2001 sequence anyone? Callum Bell
mRNA from Total RNA: Pref Methods? Michael Myers
Mouse liver DNA? Daniel L. Burgess
blunt ended ligation HARDIES at THORIN.UTHSCSA.EDU
Radioactive probes by PCR Daniel L. Burgess
lambda 2001 sequence anyone? Dave Pilgrim
mRNA from Total RNA: Pref Methods? Slawek Dabrowski
Info. on Thiopropyl-Sepharose KevO
List of neato keen home pages Paul N Hengen
cla I site in Bluescript II Rick Neubig
PCR A/T CLONING VECTOR haddad at horta
plasmid prep kits: good & bad? Rae Nishi
Green Fluo. Prot. David J Askew
cla I site in Bluescript II Klaus Salger
cycle vs. normal sequencing Mic Chaudoir
Radioactive probes by PCR Klaus Salger
help on NA45 and wais... tao at mbcf.stjude.org
PCR A/T CLONING VECTOR Paul N Hengen
Biotin Labeling cDNA probes June Eva Paciga
Differential Display of Artefacts? Shahram Mori
cycle vs. normal sequencing Karl A. Suiter
Comparison of 200 ul thin wall PCR tubes Chung-Ming Hsieh
Blunt ended ligation Ron Kagan
Need sequence of pT7-7 Zhixiang Hu
GUS staining HEAZLEWOOD,Joshua
Culture Medium? ATI3
Need sequence of pT7-7 Slawek Dabrowski
Maxipep "problem" Mark Thornton
Gel video documentation choie01 at mcrcr6.med.nyu.edu
Need sequence of pT7-7 Tracy Aquilla
[Q] Gancyclovir/FIAU Vasily Dobrovolsky
Comparison of 200 ul thin wall PCR tubes Michael Cooley
Triton in place of SDS Louis Hom
Need catalog for molecular probe K Y Hwa
RNA from rheumatoid synovial biopsy tissue Peter J. Crilly
RT-PCR Andre Hamel
"best" polymorphic CA repeat promers Varyc
human cell lines with high viability jxu at MIT.EDU
Does this strain exist? Richard_Heath
help on NA45 and wais... Klaus Salger
RNA / nothern blotting question Lana C Stallard
Bot Tox Affinity Column??? pe.ma
Drosophila nuclei prep pe.ma
Induction by macromolecs Louis Hom
deep microtiter dishes Chuck Staben
RNA from rheumatoid synovial biopsy tissue Jukka Luoma
Need catalog for molecular probe Sebastian W. Bunka
FOR BECOMING A MEMBER. cancer%bic-mku at dbt.ernet.in
reagent wangyq at BEPC2.IHEP.AC.CN
Agarose gel fluorography Dennis Templeton
Chloroform colony lysis Peter
reagent wangyq at BEPC2.IHEP.AC.CN
Induction by macromolecs NICHOLAS THEODORAKIS
T3/T$ 4/TSH/96 WELL KIT? LUMINESCE at BIOTECHNET.COM
Beta-Actin good RT-PCR Control? Sean David Moore
Beta-Actin good RT-PCR Control? Richard R. Hardy
Special methods Slawek Dabrowski
Beta-Actin good RT-PCR Control? David Huen
test for mycoplasm contamination Manfred Gessler
Non-Denaturing RPA Gels Aaron Pawlyk
1069 BIOSCI Administrator
Beta-Actin good RT-PCR Control? Richard R. Hardy
alkaline phosphatase labeled oligos lcp at VETMED.WSU.EDU
Induction by macromolecs Louis Hom
Methanococcus Duncan Clark
Blunt ended ligation Duncan Clark
Does CTAB effect 260nm readings? Mark Hicks - Genetics Group/University of Alberta

Last message date: Sat Dec 31 20:06:24 EST 1994
Archived on: Sun Mar 6 20:21:56 EST 2005

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