Pharmarcia Red Sepharose CL-6B

Ing-Nang Wang
Tue Feb 1 22:27:58 EST 1994

I wonder if anyone has experience on Pharmacia Red Sepharose CL-6B? 
Recently, I tried to partially purify 6-phosphogluconate dehydrogenase (use
NADP) from E. coli.  However, I found it very difficult to elute the enzyme
from the column.  The elution buffer I used is HEPES 50 mM + MgCl2 10 mM +
NADP from 1 to 3 mM.  Any idea on this?

Ing-Nang Wang
ingnang at

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