Small c: a 50bp PCR-products, visualization?

Timo Hiltunen hiltunen at convex.csc.FI
Wed Feb 2 05:31:00 EST 1994


In <1994Jan28.152547.7169 at alw.nih.gov> dspete at helix.nih.gov writes:


>In article <1994Jan28.105904.4894 at nomina.lu.se>, <Lars.S at Plantbio.lu.se> 
>writes:

>> 
>> I will start running some PCRs with primers derived from N/terminal amino 
>> acid sequences based on method described by Walker et.al 1992. And I am 
>> woundering if somebody knows of a good gel method to separate DNA 
>> fragments of size about 50 bp? It would be nice to be able to visulaize 
>> the PCR products separated on gel to be able to tell if a product is made 
>> in the reactions.
>> All replies or related suggestions appreciated.
>> _________________________________________________________
>> Lars Snogerup               Lars.S at Plantbio.lu.se
>> Plant Biochemistry
>> Lund University
>> Sweden

>You could try PCR Purity Plus from AT Biochem (800-282-4626, FAX 215-889-9304). 
>It's a modified acrylamide and should separate fragments as small as 50 bp. 
>Alternatively just try running the products on a 10 or 20 acrylamide gel.

>-------------------------------------------------------------------------
>* Dr. David S. Peterson             Phone  (301) 496-4023               *
>* National Institutes of Health                                         *
>* Laboratory of Malaria Research    Fax    (301) 402-0079               *
>* Bldg4, Rm B1-34                                                       *
>* Bethesda MD, 20892                Email: dspete at helix.nih.gov         *
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Hi!

MetaPhor Agarose from FMC (about 4 %) gives a very good resolution,

especially in 0.5xTBE buffer.

Timo Hiltunen, M.D.
University of Tampere
Finland




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