Erase a Base
leach at mbcrr.harvard.edu
Wed Feb 2 23:23:33 EST 1994
In article <1994Feb2.231108.22049 at vaxkiller.agi.org>,
vaxkiller.agi.org!rlynch (rick lynch) wrote:
> I need to generate sets of nested deletions. I have been looking into a kit
> sold by Promega called "Erase-a-Base" The company claims it is one of the most
> wonderful nested deletion kit on the market, but if I had a nickel for every
> time I heard that I wouldn't be working.:-)
> Anyone tryed it? If so, could you please let me know what you think of it.
Tried it, Hated it.
First screen on one of the deletions found about 3 deleted clones. When
checking for more, screened 50-60 plus other time points, found none. Even
after 3 attempts at redoing the deletions :)
Found it much quicker to make oligos and make PCR products and clone them,
or r.enz fragments and glue them together.
..... Martin Leach Email:leach at mbcrr.harvard.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants on your
USA head.....WIBBLE" -BLACKADDER
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