PCR inhibition by U.V.

Richard Near rocket1 at bu.edu
Wed Feb 2 20:16:27 EST 1994


In order to eliminate contamination in my
PCR reactions, I have treated most reagents
with uv (except the oligos) including the
wax, and am now getting poor yields where
I used to get better yields using the same 
temperatures/times.  THE question:: does uv
create some chemical products that inhibit
pcr????

Also I have heard that just storing oligo's for
long periods in water at -20 C. will result in
the oligo's being less efficient for PCR..

ANY COMENTS?
Rich
rocket1 at bu.edu




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