Lacz expression plasmids with nuclear localization signal?
bmurray at calvin.bio.uci.edu
Thu Feb 3 15:54:25 EST 1994
I'm looking for sources for eukaryotic expression plasmids carrying the
beta-galactosidase gene with and without nuclear localization signals. I
would like to use these plasmids in an upper-division (juniors and
seniors) undergraduate cell biology laboratory course to demonstrate
expression of transfected genes in cultured eukaryotic cells (probably
Balb/c 3T3's) and to show that specific peptide sequences in proteins can
give rise to drastic differences in where proteins go. Can anyone help me
out? (Replies by e-mail are fine.) Thanks!
Ben Murray, Developmental & Cell Biology, UC Irvine, Irvine, CA 92717-2300
E-mail: bamurray at uci.edu
More information about the Methods