PAGE - misformed wells - help

Darren Fast dfast at gpu.srv.ualberta.ca
Thu Feb 3 15:54:00 EST 1994


One thing that I've found to prevent misformed wells is ensuring the 
combs and plates are meticulously clean (glass plates washed with soap 
and water and rinsed with acetone, plastic spacers washed and rinsed with 
70% ethanol and wiped dry with a kim-wipe).  It is also a good idea to 
not let the wells polymerize to long usually not more than 1.5 hours. If 
you need to pour the wells and leave them overnight, remove the combs and 
fill the wells with buffer or water to prevent them from drying out.
Hope this helps



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