Vent (exo +) and RACE
vam2 at midway.uchicago.edu
Thu Feb 3 13:19:03 EST 1994
I was wondering whether anyone has performed 3' or 5' RACE using NEB
(exo+)? I wanted to use this enzyme as opposed to Taq because of the
fidelity. I'd like to hear whatever comments/experiences you may have
Currently, I am using Tfl DNA polymerase from Epicentre
I am getting a significant number of point mutations.
Additionally, has anyone done RACE without adding 10% DMSO? I was
whether the reaction is as forgiving without this addition.
Dept. of Organismal Biology and Anatomy
Univ. of Chicago
More information about the Methods