extract membrane protein

Michael Coady coady at ERE.UMontreal.CA
Fri Feb 4 13:44:50 EST 1994

In article <60632 at sdcc12.ucsd.edu> wsun at jeeves.ucsd.edu (Fiberman) writes:
>I am expressing a receptor in Xenopus oocytes.  It is a membrane
>protein.  I wish to extract the protein for a Western blot.
>Does anyone out there have a quick and simple protocol for
>extracting membrane protein from frog eggs?  I would appreciate
>your help.  Thanks.

Sure do.  Check out Wall & Patel, in J. Memb. Biol. vol 107 pp 189-201 (1989).
You're welcome.  By the way, it's an easy technique that works quite well.


Michael J. Coady
The opinions expressed above are solely those of the author and do not,
in any way, shape or form, represent the Universite de Montreal.

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