extract membrane protein

Michael Coady coady at ERE.UMontreal.CA
Fri Feb 4 13:44:50 EST 1994


In article <60632 at sdcc12.ucsd.edu> wsun at jeeves.ucsd.edu (Fiberman) writes:
>I am expressing a receptor in Xenopus oocytes.  It is a membrane
>protein.  I wish to extract the protein for a Western blot.
>Does anyone out there have a quick and simple protocol for
>extracting membrane protein from frog eggs?  I would appreciate
>your help.  Thanks.
>
>-fm
>

Sure do.  Check out Wall & Patel, in J. Memb. Biol. vol 107 pp 189-201 (1989).
You're welcome.  By the way, it's an easy technique that works quite well.

Mike

-- 
Michael J. Coady
COADY at ERE.UMONTREAL.CA
The opinions expressed above are solely those of the author and do not,
in any way, shape or form, represent the Universite de Montreal.



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