extract membrane protein
BARRY J MARGULIES
bjmarg at welchlink.welch.jhu.edu
Sun Feb 6 14:15:56 EST 1994
In article <60632 at sdcc12.ucsd.edu> wsun at jeeves.ucsd.edu (Fiberman) writes:
>I am expressing a receptor in Xenopus oocytes. It is a membrane
>protein. I wish to extract the protein for a Western blot.
>Does anyone out there have a quick and simple protocol for
>extracting membrane protein from frog eggs? I would appreciate
>your help. Thanks.
I would like to append a request: Does anyone have such a protocol for
extraction of such from *any* cell type. I've been trying for a couple
years now, using many different types of detergent/buffer combinations
for immppt. It seems that every time I turn around, there's another
option to try. Anyway, any and all help would be greatly appreciated.
-Barry (bjmarg at welchlink.welch.jhu.edu)
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