PCR inhibition by U.V.

Ron Kagan rkagan at ewald.mbi.ucla.edu
Sat Feb 5 19:34:54 EST 1994


In article <2ipj9b$hva at news.bu.edu> Richard Near, rocket1 at bu.edu writes:
>In order to eliminate contamination in my
>PCR reactions, I have treated most reagents
>with uv (except the oligos) including the
>wax, and am now getting poor yields where
>I used to get better yields using the same 
>temperatures/times.  THE question:: does uv
>create some chemical products that inhibit
>pcr????

I irradiate my PCR reactions all of the time with no problems.  I
irradiate the reaction mixtures containing everything *except* the
template, the oligos, and the Taq.  These, I think, are the only
components that would be affected by UV.

Ron Kagan
rkagan at ewald.mbi.ucla.edu



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