TA cloning system
daj (David Johnston)
daj at nhm.ic.ac.uk
Mon Feb 7 03:14:28 EST 1994
On 6 Feb 1994 08:41:45 -0000,
Eric Yang writes:
>Could anyone out there tell me something about the TA cloning system
>(Invitrogen) for cloning PCR products? What are it's advantages and
>disadvantages? Does it really work?
Yep, it sure does (for us anyway). The new version is better than the
original as it uses ampicillin selection rather than kanomycin so you
don't have to make yet another type of plate. The only dissadvantages we
have found are (1) copy number isn't particularly high (2) you get
mountains of legal paperwork with the kit which you are suppossed to sign
anmd return, swearing on your granny's grave that you won't give any of
thre plasmid away etc..
Promega do a pGem T kit which does the same thing, works well in our
hands, has a higher copy number, uses ampicillin selection and JM109 cells
(our lab standard so no worries if the supplied competent cells run out -
TA cloning uses INF alpha cells). Worth considering also.
David A. Johnston
Dept of Zoology, The Natural History Museum, Cromwell Road,
South Kensington, London SW7 5DB.
(tel 071 9389297, fax 071 9388754, email daj at nhm.ic.ac.uk)
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