Detection of Glycosylated Proteins !

Bullock, David bullock at kahu.lincoln.ac.nz
Mon Feb 7 20:47:04 EST 1994


In article <mdhouse-060294125020 at alliance.hip.berkeley.edu> mdhouse at mendel.berkeley.edu () writes:
>From: mdhouse at mendel.berkeley.edu ()
>Subject: Re: Detection of Glycosylated Proteins !
>Date: Sun, 06 Feb 1994 12:50:19 -0800

>In article <2j0boj$7or at mserv1.dl.ac.uk>, jawahar at nii.ernet.in wrote:

>> Hello,
>> Does any one have a method for detecting glycosylated proteins 
>> on nitrocellulose membrane by any other method other than silver
>> staining..
>> I am currently using Schiff's reagent but the levels of detections
>> are very low.. Can anyone suggest an improvement over this too !

>Try using 125I - labelled ConA as detection method. ConA will recognize
>most glycoproteins on nitrocellulose. Furthermore it will be fairly
>sensitive and quantifyable.
>Good luck, Martin

You could also try VECTASTAIN kits from Vector Labs which we have used very 
successfully in the past.  These are avidin/biotin based systems that offer 
an amplified signal with a variety of lectin probes and improved sensitivity 
over 125I without the hassle of labelling or isotope use.




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