Large Scale Plasmid DNA Restriction Endonuclease Digest

weston at skyfox.usask.ca weston at skyfox.usask.ca
Tue Feb 8 10:25:31 EST 1994


I have a large quantity of plasmid (pUC deriviative) DNA to digest
with :
1) EcoRI
2) EcoRI and HindIII double digest.
What is the largest amount of plasmid DNA that anyone has digested at any one
time (one reaction tube)?  I have approximately one milligram of purified
plasmid DNA which needs to be restricted in as short a time as possible.
Thanks in advance.

        Brigitte Weston.



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