drying polyacrylamide gels

Sudhakar LPBABU at WEIZMANN.weizmann.ac.il
Thu Feb 10 10:33:05 EST 1994


Dear netters,

I am using 10-20% linear gradient polyacrylamide gels for the separation of
polypeptides. After the run, I treat my gel with 30% methanol and 3% glycerol
to give stability for the gel while drying. All I want to know now is: If I
do not wash the gel after this treatment and go for drying directly, will the
methanol cause any damage to the gel drier or to the vacuum pump? I shall be
thankful if somebody can give suggestions on this.

Sudhakar.                 e.mail:-  lpbabu at weizmann.weizmann.ac.il



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