RNA storage

Andre Hamel hamel at cc.umanitoba.ca
Thu Feb 10 17:20:28 EST 1994

Chomczynski, P. 1992. Solubilization in formamide protects RNA from
degradation. Nucl. Acids Res. 20 (14): 3791-3792.

BTW, suggested for RNAs of known conc ... "ready for gel" (add EtBr/dye
mix,  heat 70*C/10 min -> ice -> load

Have extracted RNA from cultured cells in 10x vol formamide -> vortex ->
microfuge 5 min/4*C -> save sup -> ready for RT-PCR, gel, etc

For RT-PCR, found that Vent, Deep Vent (Pfu) DNA pols tolerate up to 20%
formamide conc, whereas Taq can handle only about 5%.

Great method for quick DNA prep too (for PCR) ... there's been several
publications on use of formamide for DNA ext'n from whole blood for
example (NAR, Biotechniques).

best regards,
Andre Hamel                                    email: hamel at cc.umanitoba.ca
Manitoba Government Veterinary Services          lab tel.: (204) 945-7630
545 University Crescent,                              FAX: (204) 945-8062 
Winnipeg, Manitoba, 
CANADA   R3T 5S6            ********************

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