Blunt end ligations

botjlh at lure.latrobe.edu.au botjlh at lure.latrobe.edu.au
Sun Feb 13 07:57:03 EST 1994


In article <9402081412.AA10064 at polywog.biology.uofs.edu>, greuelb1 at jaguar.uofs.edu (Brian T. Greuel) writes:
>> 
>> STUFF DELETED
>>
>> Now I would like to know how you usually perform these reactions at the 
>> way that they work? I would appreciate any good advice or hint to make 
>> these simple things to work... 
>> 
>> Thanks, 
>> Marko Laine 
> 
> Marko, the standard approach for 3' overhangs is just what you mentioned:  
> chew back with T4 DNA polymerase in the presence of dNTPs.  It works well in 
> my hands...
> 
> Brian Greuel
> University of Scranton
> Scranton, PA

That goes 'ditto' for me.

Joshua Heazlewood
botjlh at lure.latrobe.edu.au
La Trobe University
Australia



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