Fuzzy Sequencing Gel Bands

schoenfeld at MSSCC.MED.UTAH.EDU schoenfeld at MSSCC.MED.UTAH.EDU
Mon Feb 14 18:37:43 EST 1994


Hi -
Can anyone tell me why I'm getting fuzzy bands on sequencing gels?  For
each sample, I usually do 2.5 hr and 6 hr runs side by side.  The 2.5 hr
run looks fine, but the 6 hr run is fuzzy.  Since they're on the same gel,
I don't think the fuzziness is due to something like gel temperature or
buffer depletion. Any ideas?


Thanks
Robert




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