Ligation Temperature

Steve Rodems smrodems at students.wisc.edu
Tue Feb 15 19:53:00 EST 1994


In article <2jreh2$b1d at charm.magnus.acs.ohio-state.edu>,
lritter at magnus.acs.ohio-state.edu (Linda M Ritter) wrote:

> 
> Our lab does ligations at room temperature (2 hours to overnight) and they work
> quite well.
> 
> I have not heard anything about 37 degree ligations.

Same here.  I often do 1 hour ligations when I'm in a hurry but 2 hours is
standard.

-- 
Steve "Some day I will get the hell out of Wisconsin" Rodems

"You know, the other day I ... oh, wait, that wasn't me"



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